Tóm tắt

Objectives: Establishing an HPLC method that is suited with current conditions of the laboratory to quantify 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) in dry extract of Radix Fallopiae multiflorae. Material and methods: Parameters of the method are validated including: linear range, precision, accuracy... Results: Chromatographic conditions of the method: using Sunfire RP-C18 column, acetonitrile and 0.1% aqueous formic acid as mobile phase following a multistep gradient program, monitored at 320 nm. The established method has good precision (RSD = 2.37%) and accuracy (recovery ratio = 97.08 - 103.76%, average = 100.38%, RSD = 2.84%). Conclusion: HPLC method for quantifying THSG in dry extract of Radix Fallopiae multiflorae has been developed and validated. * Key words: Fallopia multiflora; 2,3,5,4’-tetrahydroxystilben-2-O-β-D-glucosid; HPLC method.

Abstract

Objectives: Establishing an HPLC method that is suited with current conditions of the laboratory to quantify 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) in dry extract of Radix Fallopiae multiflorae. Material and methods: Parameters of the method are validated including: linear range, precision, accuracy... Results: Chromatographic conditions of the method: using Sunfire RP-C18 column, acetonitrile and 0.1% aqueous formic acid as mobile phase following a multistep gradient program, monitored at 320 nm. The established method has good precision (RSD = 2.37%) and accuracy (recovery ratio = 97.08 - 103.76%, average = 100.38%, RSD = 2.84%). Conclusion: HPLC method for quantifying THSG in dry extract of Radix Fallopiae multiflorae has been developed and validated. * Key words: Fallopia multiflora; 2,3,5,4’-tetrahydroxystilben-2-O-β-D-glucosid; HPLC method.