Tóm tắt

Objective: To develop and evaluate the analgesic effects of the tincture from the Momordica cochinchinensis seeds - Ramulus cinnamomi. Methods: The Momordica cochinchinensis seeds were extracted by the Soxhlet method. Total saponins were determined by UV-Vis spectrophotometry after extracts had been colored with perchloric acid, vanillin in glacial acetic acid. Ramulus cinnamomi were extracted by the percolation. The tincture was made by combining the extract of the Momordica cochinchinensis seeds and Ramulus cinnamomi. The skin irritation of tincture was evaluated in rabbits. The analgesic effects of tincture were evaluated in rats in hot plate model with the use of pain agent carragenaan. Results: The Soxhlet extraction in 16 hours by 70% ethanol solvent was the highest total saponins content (about 13.53%). The low rate of Ramulus cinnamomi in the tincture made it very unpleasant. Their skin irritation was proportional to the amount of Ramulus cinnamomi. Evaluation of analgesic effect showed that the tincture was capable of reducing pain on the hot-plate model. Conclusion: The tincture that ratio 16.7% of Ramulus cinnamomi had good quality and unirritated the skin and had analgesic effect on the experimental model. * Keywords: Momordica cochinchinensis seed; Ramulus cinnamomi; Tincture; Irritation, Analgesic.

Abstract

Objective: To develop and evaluate the analgesic effects of the tincture from the Momordica cochinchinensis seeds - Ramulus cinnamomi. Methods: The Momordica cochinchinensis seeds were extracted by the Soxhlet method. Total saponins were determined by UV-Vis spectrophotometry after extracts had been colored with perchloric acid, vanillin in glacial acetic acid. Ramulus cinnamomi were extracted by the percolation. The tincture was made by combining the extract of the Momordica cochinchinensis seeds and Ramulus cinnamomi. The skin irritation of tincture was evaluated in rabbits. The analgesic effects of tincture were evaluated in rats in hot plate model with the use of pain agent carragenaan. Results: The Soxhlet extraction in 16 hours by 70% ethanol solvent was the highest total saponins content (about 13.53%). The low rate of Ramulus cinnamomi in the tincture made it very unpleasant. Their skin irritation was proportional to the amount of Ramulus cinnamomi. Evaluation of analgesic effect showed that the tincture was capable of reducing pain on the hot-plate model. Conclusion: The tincture that ratio 16.7% of Ramulus cinnamomi had good quality and unirritated the skin and had analgesic effect on the experimental model. * Keywords: Momordica cochinchinensis seed; Ramulus cinnamomi; Tincture; Irritation, Analgesic.