Tóm tắt

Objectives: To evaluate the effects of high-fat diet (HFD) consumption on locomotion, exploration, and object recognition in rats. Subjects and methods: 62 white male rats (8 - 9 weeks old) were caged individually and randomly divided into two groups by the diets of high-fat and standard food (control group) ones for 7 consecutive weeks. Their behaviors were then assessed with a battery of tests for locomotion and exploration in an open-field apparatus, and a test for novel object recognition. Results: HFD fed rats reduced the length and duration of exploration within the open-field in comparison to those in the control group (trajectory length: Control group: 1800.24 ± 438.92 cm; HFD group: 1317.29 ± 566.10 cm; p < 0.001; duration: Control group: 215.25 ± 32.38s; HFD group: 174.35 ± 67.34s; p = 0.004) and lowered the exploratory activity to the open-field’s central zone (trajectory length: Control group: 91.09 ± 47.76 cm; HFD group: 64.51± 56.58 cm; p = 0.05; duration: Control group: 8.94 ± 6.67s; HFD group: 7.03 ± 5.74s; p = 0.23). Rats bred with HFD showed a deficit of short-term memory via novel object recognition test (object exploration spent time ratios, expressed as familiar and novel objects consequently: Control group: 41.29 ± 17.65% and 58.71 ± 17.65%, p < 0.001; HFD group: 50.32 ± 16.89% and 49.68 ± 16.89%; p = 0.88). Conclusions: Rats fed with HFD showed less locomotor and exploratory activities, more anxiety-like behavior in the open-field, and a defected short-term memory compared to their peer consumed standard food. * Keywords: High-fat diet; Locomotion; Exploration; Object recognition; Rats.

Abstract

Objectives: To evaluate the effects of high-fat diet (HFD) consumption on locomotion, exploration, and object recognition in rats. Subjects and methods: 62 white male rats (8 - 9 weeks old) were caged individually and randomly divided into two groups by the diets of high-fat and standard food (control group) ones for 7 consecutive weeks. Their behaviors were then assessed with a battery of tests for locomotion and exploration in an open-field apparatus, and a test for novel object recognition. Results: HFD fed rats reduced the length and duration of exploration within the open-field in comparison to those in the control group (trajectory length: Control group: 1800.24 ± 438.92 cm; HFD group: 1317.29 ± 566.10 cm; p < 0.001; duration: Control group: 215.25 ± 32.38s; HFD group: 174.35 ± 67.34s; p = 0.004) and lowered the exploratory activity to the open-field’s central zone (trajectory length: Control group: 91.09 ± 47.76 cm; HFD group: 64.51± 56.58 cm; p = 0.05; duration: Control group: 8.94 ± 6.67s; HFD group: 7.03 ± 5.74s; p = 0.23). Rats bred with HFD showed a deficit of short-term memory via novel object recognition test (object exploration spent time ratios, expressed as familiar and novel objects consequently: Control group: 41.29 ± 17.65% and 58.71 ± 17.65%, p < 0.001; HFD group: 50.32 ± 16.89% and 49.68 ± 16.89%; p = 0.88). Conclusions: Rats fed with HFD showed less locomotor and exploratory activities, more anxiety-like behavior in the open-field, and a defected short-term memory compared to their peer consumed standard food. * Keywords: High-fat diet; Locomotion; Exploration; Object recognition; Rats.